PRODUCT CODE: ET1603-41

Recombinant Phospho P-V-Myb+C-Myb (S11) Monoclonal Antibody (ET1603-41)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

ICC staining P-V-Myb+C-Myb(S11) in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining P-V-Myb+C-Myb(S11) in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human uterine neck tissue using anti-P-V-Myb+C-Myb(S11) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-P-V-Myb+C-Myb(S11) antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with P-V-Myb+C-Myb(S11) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as antibody
ICC staining P-V-Myb+C-Myb(S11) in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Phospho P-V-Myb+C-Myb (S11) Monoclonal Antibody (ET1603-41)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser11 of human v-myb+c-myb.

Host

Rabbit

Modification

Phospho

Modification Site

S11

Positive Control

Ags, human uterine neck tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SZ04-81

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

72/75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

P-V-Myb+C-Myb(S11)

SYNONYMS

C myb antibody; C myb protein antibody; Cmyb antibody; MYB antibody; Myb proto oncogene antibody; V myb myeloblastosis viral oncogene homolog antibody

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.; Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.; Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The Myb family of transcription factors regulates proliferation, differentiation and apoptosis of hematopoletic cells. The avian myeloblastosis viral (v-Myb) protein is nuclear and binds to specific DNA sequences. The gene encoding for v-Myb is oncogenic, causing monoblastic leukemia and transforming myelomonocytic cells by deregulating the expression of specific target genes. v-Myb functions as a transcriptional activator, and it can repress biologically relevant genes such as Ets-2, which promotes macrophage differentiation. The proto-oncogene c-Myb encodes for a nuclear protein that plays a role in transcriptional regulation and may be essential for hematopoietic cell proliferation. A-Myb is also member of the Myb family that is expressed in proliferating B cell centroblasts. Transgenic mice overexpressing A-Myb posses enhanced hyperplasia of the lymph nodes.