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PRODUCT CODE: ET1612-42

Recombinant Phospho MEK1 (T292) Monoclonal Antibody (ET1612-42)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

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Western blot analysis of Phospho-MEK1 (T292) on Daudi cells lysates using anti-Phospho-MEK1 (T292) antibody at 1/1,000 dilution.
  • Western blot analysis of Phospho-MEK1 (T292) on Daudi cells lysates using anti-Phospho-MEK1 (T292) antibody at 1/1,000 dilution.
Western blot analysis of Phospho-MEK1 (T292) on Daudi cells lysates using anti-Phospho-MEK1 (T292) antibody at 1/1,000 dilution.

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Phospho MEK1 (T292) Monoclonal Antibody (ET1612-42)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding thr292 of human mek1

Host

Rabbit

Modification

Phospho

Modification Site

T292

Positive Control

Daudi.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD2088

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

Q02750

PROTEIN NAME

Phospho-MEK1 (T292)

SYNONYMS

Dual specificity mitogen activated protein kinase kinase 1 antibody; Dual specificity mitogen-activated protein kinase kinase 1 antibody; ERK activator kinase 1 antibody; MAP kinase kinase 1 antibody; MAP kinase/Erk kinase 1 antibody; MAP2K1 antibody; MAPK/ERK kinase 1 antibody; MAPKK 1 antibody; MAPKK1 antibody; MEK 1 antibody; Mek1 antibody; MEKK1 antibody; Mitogen activated protein kinase kinase 1 antibody; MKK 1 antibody; MKK1 antibody; MP2K1_HUMAN antibody; PRKMK1 antibody; Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody; Protein kinase mitogen activated, kinase 1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

TISSUE SPECIFICITY

Widely expressed, with extremely low levels in brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation.; Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Membrane.

FUNCTION

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.