Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lane 2: 293T cell lysate
Recombinant Rabbit monoclonal primary
Recombinant Phospho MEK1 (S218/S222) Monoclonal Antibody (ET1609-50)
Synthetic phospho-peptide corresponding to residues surrounding ser218 and 222 of human mek1.
Hela cell lysate, A431 cell lysate, 293T cell lysate, Hela, HepG2, NIH/3T3, human tonsil tissue, human liver carcinoma tissue, human spleen tissue, human breast carcinoma tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
Dual specificity mitogen activated protein kinase kinase 1 antibody; Dual specificity mitogen-activated protein kinase kinase 1 antibody; ERK activator kinase 1 antibody; MAP kinase kinase 1 antibody; MAP kinase/Erk kinase 1 antibody; MAP2K1 antibody; MAPK/ERK kinase 1 antibody; MAPKK 1 antibody; MAPKK1 antibody; MEK 1 antibody; Mek1 antibody; MEKK1 antibody; Mitogen activated protein kinase kinase 1 antibody; MKK 1 antibody; MKK1 antibody; MP2K1_HUMAN antibody; PRKMK1 antibody; Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody; Protein kinase mitogen activated, kinase 1 antibody; hide
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Widely expressed, with extremely low levels in brain.
Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation.; Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Cytoplasm, Nucleus, Membrane.
Activation of extracellular signal-regulated kinase (ERK) or mitogen-activated protein kinase by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ERKs. MEK1 and MEK2 can also be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK activator MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants.