PRODUCT CODE: ET1701-19

Recombinant Phospho Hsp27(S78) Monoclonal Antibody (ET1701-19)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of Phospho-Hsp27(S78) on different lysates using anti-Phospho-Hsp27(S78) antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Human heart <br />
 Lane 2: Human skeletal muscle
  • Western blot analysis of Phospho-Hsp27(S78) on different lysates using anti-Phospho-Hsp27(S78) antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Human heart <br />
 Lane 2: Human skeletal muscle
  • ICC staining Phospho-Hsp27(S78) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-Hsp27(S78) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-Hsp27(S78) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Hsp27(S78) antibody. Counter stained with hematoxylin.
Western blot analysis of Phospho-Hsp27(S78) on different lysates using anti-Phospho-Hsp27(S78) antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human heart
Lane 2: Human skeletal muscle

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Phospho Hsp27(S78) Monoclonal Antibody (ET1701-19)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser78 of human hsp27.

Host

Rabbit

Modification

Phospho

Modification Site

S78

Positive Control

NIH/3T3, MCF-7, Hela, human breast carcinoma tissue, human heart tissue, human skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ08-70

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

27 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Heat shock protein beta-1

GENE NAME

HSPB1

SYNONYMS

HspB1, HSP 27, SRP27, HSPB1

SEQUENCE SIMILARITIES

Belongs to the small heat shock protein (HSP20) family.

TISSUE SPECIFICITY

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

FUNCTION

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.