Positive control:
Lane 1: mouse spleen tissue lysate
Lane 2: rat spleen tissue lysate
Applications
-
WB
-
ICC
-
IHC-P
-
IP
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Phospho eIF4E (S209) Monoclonal Antibody (ET1608-66)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser209 of human eif4e.
Host
Rabbit
Modification
Phospho
Modification Site
S209
Positive Control
Mouse spleen tissue lysate, rat spleen tissue lysate, N2A, SH-SY5Y, 293, human spleen tissue, rat spleen tissue, mouse spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU0396
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
25 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Phospho-eIF4E (S209)
SYNONYMS
AUTS19 antibody; CBP antibody; eIF 4E antibody; eIF 4F 25 kDa subunit antibody; EIF 4F antibody; eIF-4E antibody; eIF-4F 25 kDa subunit antibody; eIF4E antibody; EIF4E1 antibody; EIF4EL1 antibody; EIF4F antibody; Eukaryotic translation initiation factor 4 E antibody; Eukaryotic translation initiation factor 4E antibody; Eukaryotic translation initiation factor 4E like 1 antibody; IF4E_HUMAN antibody; Messanger RNA Cap Binding Protein eIF 4E antibody; MGC111573 antibody; mRNA cap binding protein antibody; mRNA cap-binding protein antibody
SEQUENCE SIMILARITIES
Belongs to the eukaryotic initiation factor 4E family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.