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PRODUCT CODE: ET1612-31

Recombinant Phospho Cyclin E1(T77) Monoclonal Antibody (ET1612-31)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

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ICC staining Phospho-Cyclin E1(T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-Cyclin E1(T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-Cyclin E1(T77) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-Cyclin E1(T77) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Cyclin E1(T77) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Cyclin E1(T77) antibody. Counter stained with hematoxylin.
ICC staining Phospho-Cyclin E1(T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Phospho Cyclin E1(T77) Monoclonal Antibody (ET1612-31)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding thr77 of human cyclin e1.

Host

Rabbit

Modification

Phospho

Modification Site

T77

Positive Control

SW480, Hela, HepG2, human tonsil tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD2025

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P24864

PROTEIN NAME

G1/S-specific cyclin-E1

GENE NAME

CCNE1

SYNONYMS

CCNE1

SEQUENCE SIMILARITIES

Belongs to the cyclin family. Cyclin E subfamily.

TISSUE SPECIFICITY

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.; Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Essential for the control of the cell cycle at the G1/S (start) transition.