Applications
-
WB
-
ICC
-
IF
-
IHC-P
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Phospho Cyclin E1 (T77) Monoclonal Antibody (ET1612-31)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding thr77 of human cyclin e1.
Host
Rabbit
Modification
Phospho
Modification Site
T77
Positive Control
SW480, Hela, HepG2, human tonsil tissue, human colon carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD2025
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
48 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Cyclin E1(T77)
SYNONYMS
CCNE antibody; Ccne1 antibody; CCNE1_HUMAN antibody; cyclin E variant ex5del antibody; cyclin E variant ex7del antibody; Cyclin E1 antibody; Cyclin Es antibody; Cyclin Et antibody; CyclinE antibody; G1/S specific cyclin E antibody; G1/S-specific cyclin-E1 antibody
SEQUENCE SIMILARITIES
Belongs to the cyclin family. Cyclin E subfamily.
TISSUE SPECIFICITY
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.; Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.