Positive control:
Lane 1: Hela
Lane 2: Mouse spleen
Lane 3: NIH/3T3
Applications
-
WB
-
IHC-P
-
IP
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Phospho Cdk2 (Y15) Monoclonal Antibody (ET1611-52)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding tyr15 of human cdk2.
Host
Rabbit
Modification
Phospho
Modification Site
Y15
Positive Control
Hela cell lysate, mouse spleen tissue lysate, NIH/3T3 cell lysate, human breast carcinoma tissue, mouse colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN72-04
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
34 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Cdk2(Y15)
SYNONYMS
Cdc2 related protein kinase antibody; cdc2-related protein kinase antibody; CDC28 antibody; CDC2A antibody; Cdk 2 antibody; CDK1 antibody; CDK2 antibody; CDK2_HUMAN antibody; CDKN2 antibody; Cell devision kinase 2 antibody; Cell division protein kinase 2 antibody; Cyclin dependent kinase 2 antibody; cyclin dependent kinase 2-alpha antibody; Cyclin-dependent kinase 2 antibody; kinase Cdc2 antibody; MPF antibody; p33 protein kinase antibody; p33(CDK2) antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A.; Nitrosylated after treatment with nitric oxide (DETA-NO).
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Endosome.
FUNCTION
In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE.