PRODUCT CODE: ET1612-96

Recombinant Phospho Cdc6 (S54) Monoclonal Antibody (ET1612-96)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

Western blot analysis of Phospho-Cdc6(S54) on Jurkat cells lysates using anti-Phospho-Cdc6(S54) antibody at 1/1,000 dilution.
  • Western blot analysis of Phospho-Cdc6(S54) on Jurkat cells lysates using anti-Phospho-Cdc6(S54) antibody at 1/1,000 dilution.
  • ICC staining Phospho-Cdc6(S54) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-Cdc6(S54) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-Cdc6(S54) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Phospho-Cdc6(S54) on Jurkat cells lysates using anti-Phospho-Cdc6(S54) antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Phospho Cdc6 (S54) Monoclonal Antibody (ET1612-96)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser54 of human cdc6.

Host

Rabbit

Modification

Phospho

Modification Site

S54

Positive Control

A431, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-47

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

63 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cell division control protein 6 homolog

GENE NAME

CDC6

SYNONYMS

HsCdc18, HsCDC6, CDC6

SEQUENCE SIMILARITIES

Belongs to the CDC6/cdc18 family.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells.

FUNCTION

Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.

CITATIONS

  • Lei, Tingjun et al.

    Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation. | Nature Communications [2018]