PRODUCT CODE: ET1604-5

Recombinant PHD1/prolyl hydroxylase Monoclonal Antibody (ET1604-5)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PHD1 on different lysates using anti-PHD1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: PC12 <br />
  Lane 3: NIH/3T3
  • Western blot analysis of PHD1 on different lysates using anti-PHD1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: PC12 <br />
  Lane 3: NIH/3T3
  • ICC staining PHD1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PHD1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PHD1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHD1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PHD1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PHD1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with PHD1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of PHD1 on different lysates using anti-PHD1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: PC12
Lane 3: NIH/3T3

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PHD1/prolyl hydroxylase Monoclonal Antibody (ET1604-5)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, SKOV-3, Hela, PC12, NIH/3T3, mouse prostate tissue, human lung cancer tissue, human breast carcinoma tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SP00-48

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

44 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PHD1/prolyl hydroxylase

SYNONYMS

DKFZp434E026 antibody; EGL nine (C.elegans) homolog 2 antibody; Egl nine homolog 2 (C. elegans) antibody; Egl nine homolog 2 antibody; EGLN 2 antibody; EGLN2 antibody; EGLN2_HUMAN antibody; EIT 6 antibody; EIT6 antibody; Estrogen-induced tag 6 antibody; HIF P4H 1 antibody; HIF PH1 antibody; HIF prolyl hydroxylase 1 antibody; HIF-PH1 antibody; HIF-prolyl hydroxylase 1 antibody; HIFPH 1 antibody; HIFPH1 antibody; HPH 3 antibody; HPH-1 antibody; HPH-3 antibody; HPH3 antibody; Hypoxia inducible factor prolyl hydroxylase 1 antibody; Hypoxia-inducible factor prolyl hydroxylase 1 antibody; P4H1 antibody; PHD 1 antibody; PhD1 antibody; prolyl hydroxylase domain containing protein 1 antibody; Prolyl hydroxylase domain-containing protein 1 antibody

TISSUE SPECIFICITY

Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by SIAH1 and/or SIAH2 in response to the unfolded protein response (UPR), leading to its degradation.

SUBCELLULAR LOCATION

Nucleus, cytosol.

FUNCTION

Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1, PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-α subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-α proteins. HIF PHD1, which is widely expressed, with highest levels of expression in testis, functions as a cellular oxygen sensor and is important in cell growth regulation. HIF PHD1 can localize to the nucleus or the cytoplasm and is also detected in hormone responsive tissues, such as normal and cancerous mammary, ovarian and prostate epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3. HIF PHD2 is regarded as the main cellular oxygen sensor, as RNA interference against HIF PHD2, but not HIF PHD1 or HIF PHD3, is enough to stabilize HIF-1α in normoxia. HIF PHD2, a direct HIF target gene, is expressed mainly in skeletal muscle, heart, kidney and brain. HIF PHD3 may play a role in the regulation of cell growth in muscle cells and in apoptosis in neuronal tissue. HIF PHD3 is widely expressed, although the highest levels can be detected in placenta and he