PRODUCT CODE: ET1702-83

Recombinant PGP9.5 Monoclonal Antibody (ET1702-83)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining PGP9.5 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PGP9.5 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PGP9.5 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PGP9.5 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
ICC staining PGP9.5 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PGP9.5 Monoclonal Antibody (ET1702-83)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-3M, Hela, PC-12, human pancreas tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF93-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PGP9.5

SYNONYMS

Epididymis luminal protein 117 antibody; Epididymis secretory protein Li 53 antibody; HEL 117 antibody; HEL S 53 antibody; NDGOA antibody; Neuron cytoplasmic protein 9.5 antibody; OTTHUMP00000218137 antibody; OTTHUMP00000218139 antibody; OTTHUMP00000218140 antibody; OTTHUMP00000218141 antibody; Park 5 antibody; PARK5 antibody; PGP 9.5 antibody; PGP9.5 antibody; PGP95 antibody; Protein gene product 9.5 antibody; Ubiquitin C terminal esterase L1 antibody; Ubiquitin C terminal hydrolase antibody; Ubiquitin C terminal hydrolase L1 antibody; Ubiquitin carboxyl terminal esterase L1 antibody; Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody; Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody; Ubiquitin thioesterase L1 antibody; Ubiquitin thiolesterase antibody; Ubiquitin thiolesterase L1 antibody; UCH-L1 antibody; UCHL1 antibody; UCHL1_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C12 family.

TISSUE SPECIFICITY

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated.

SUBCELLULAR LOCATION

Cytoplasm, Endoplasmic reticulum membrane.

FUNCTION

UCH-L1 (ubiquitin C-terminal hydrolase) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. UCH-L1 is expressed in brain neurons. Examination of specific brain regions reveals expression in all areas tested, particularly in the substantia nigra. UCH-L1 represents 1-2% of total soluble brain protein. Its occurrence in Lewy bodies and its function in the proteasome pathway make it a compelling candidate gene in Parkinson disease. The gene which encodes UCH-L1 maps to human chromosome 4p13. The 230 amino acid human UCH-L3 protein is 54% identical to that of UCH-L1. UCH-L3 is the predominant thiol protease and has high-affinity binding sites for ubiquitin.