Lane 1: Hela
Lane 2: PC-12
Lane 3: A431
Recombinant Rabbit monoclonal primary
Recombinant PFKFB3 Monoclonal Antibody (ET1705-66)
Hela cell lysate, PC-12 cell lysate, A431 cell lysate, A549, Hela, rat kidney tissue, mouse colon tissue, A431.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody; 6-bisphosphatase antibody; 6-P2ase 3 antibody; 6-P2ASE brain/placenta-type isozyme antibody; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme antibody; 6PF 2-K/Fru 2,6 P2ase 3 antibody; 6PF-2-K/Fru-2 antibody; F263_HUMAN antibody; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase antibody; Fructose-2 antibody; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase antibody; iPFK 2 antibody; iPFK-2 antibody; IPFK2 antibody; PFK/FBPase 3 antibody; PFK2 antibody; PFKFB3 antibody; Renal carcinoma antigen NY REN 56 antibody; Renal carcinoma antigen NY-REN-56 antibody; uPFK 2 antibody
In the C-terminal section; belongs to the phosphoglycerate mutase family.
Phosphorylation by AMPK stimulates activity.
Among the enzymes playing role in glycolysis,four allosteric PFKFB enzymes 1–4 expressed by four independent PFKFB genes, catalyze the rate-limiting phosphorylation of fructose-6-phosphate to fructose-1, 6-bisphosphate, using ATP as the energy source in the glycolysis pathway. Among these four allosteric enzymes, PFKFB3 enzyme retains the highest Kinase/Biphosphatase activity ratio and is expressed by PFKFB3 gene which has been demonstrated to be highly expressed in leukemic cells and in solid tumors. Moreover, mitogenic, hypoxic and inflammatory conditions have an inductive effect on the expression of PFKFB3. Hence upregulation of PFKFB genes specific to cancer cells compared to their normal counterparts (from the same patients) with more robust over-expression in breast and lung cancer make it a more appropriate target.