PRODUCT CODE: ET1705-66

Recombinant PFKFB3 Monoclonal Antibody (ET1705-66)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PFKFB3 on different cell lysates using anti-PFKFB3 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: PC-12<br />
 Lane 3: A431
  • Western blot analysis of PFKFB3 on different cell lysates using anti-PFKFB3 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: PC-12<br />
 Lane 3: A431
  • ICC staining PFKFB3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PFKFB3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PFKFB3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PFKFB3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with PFKFB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of PFKFB3 on different cell lysates using anti-PFKFB3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: A431

Applications

  • WB

  • ICC

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PFKFB3 Monoclonal Antibody (ET1705-66)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, PC-12 cell lysate, A431 cell lysate, A549, Hela, rat kidney tissue, mouse colon tissue, A431.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM43-43

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PFKFB3

SYNONYMS

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody; 6-bisphosphatase antibody; 6-P2ase 3 antibody; 6-P2ASE brain/placenta-type isozyme antibody; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme antibody; 6PF 2-K/Fru 2,6 P2ase 3 antibody; 6PF-2-K/Fru-2 antibody; F263_HUMAN antibody; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase antibody; Fructose-2 antibody; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase antibody; iPFK 2 antibody; iPFK-2 antibody; IPFK2 antibody; PFK/FBPase 3 antibody; PFK2 antibody; PFKFB3 antibody; Renal carcinoma antigen NY REN 56 antibody; Renal carcinoma antigen NY-REN-56 antibody; uPFK 2 antibody

SEQUENCE SIMILARITIES

In the C-terminal section; belongs to the phosphoglycerate mutase family.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by AMPK stimulates activity.

SUBCELLULAR LOCATION

Cytosol. Nucleoplasm.

FUNCTION

Among the enzymes playing role in glycolysis,four allosteric PFKFB enzymes 1–4 expressed by four independent PFKFB genes, catalyze the rate-limiting phosphorylation of fructose-6-phosphate to fructose-1, 6-bisphosphate, using ATP as the energy source in the glycolysis pathway. Among these four allosteric enzymes, PFKFB3 enzyme retains the highest Kinase/Biphosphatase activity ratio and is expressed by PFKFB3 gene which has been demonstrated to be highly expressed in leukemic cells and in solid tumors. Moreover, mitogenic, hypoxic and inflammatory conditions have an inductive effect on the expression of PFKFB3. Hence upregulation of PFKFB genes specific to cancer cells compared to their normal counterparts (from the same patients) with more robust over-expression in breast and lung cancer make it a more appropriate target.