PRODUCT CODE: ET1704-92

Recombinant Peroxiredoxin 3 Monoclonal Antibody (ET1704-92)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of Peroxiredoxin 3 on different cell lysate using anti-Peroxiredoxin 3 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Human liver <br />
Lane 2: MCF-7 <br />
Lane 3: A431
  • Western blot analysis of Peroxiredoxin 3 on different cell lysate using anti-Peroxiredoxin 3 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Human liver <br />
Lane 2: MCF-7 <br />
Lane 3: A431
  • ICC staining Peroxiredoxin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Peroxiredoxin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Peroxiredoxin 3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with Peroxiredoxin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Peroxiredoxin 3 on different cell lysate using anti-Peroxiredoxin 3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human liver
Lane 2: MCF-7
Lane 3: A431

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Peroxiredoxin 3 Monoclonal Antibody (ET1704-92)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human liver tissue lysate, MCF-7, A431, Hela, HepG2, human liver cancer, human kidney.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA53-21

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Thioredoxin-dependent peroxide reductase, mitochondrial

GENE NAME

PRDX3

SYNONYMS

AOP-1, Prx-III, PRDX3, AOP1

SEQUENCE SIMILARITIES

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by LRRK2; phosphorylation reduces perodixase activity.; The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.

SUBCELLULAR LOCATION

Mitochondrion. Early endosome. Note=Localizes to early endosomes in a RPS6KC1-dependent manner.

FUNCTION

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.