PRODUCT CODE: ET1704-65

Recombinant Periostin Monoclonal Antibody (ET1704-65)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of Periostin on human placenta tissue lysate using anti-Periostin antibody at 1/1,000 dilution.
  • Western blot analysis of Periostin on human placenta tissue lysate using anti-Periostin antibody at 1/1,000 dilution.
  • Flow cytometric analysis of 293T cells with Periostin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Periostin on human placenta tissue lysate using anti-Periostin antibody at 1/1,000 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Periostin Monoclonal Antibody (ET1704-65)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta tissue lysate, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA63-40

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

89 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Periostin

GENE NAME

POSTN

SYNONYMS

PN, OSF-2, POSTN, OSF2

TISSUE SPECIFICITY

Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.

POST-TRANSLATIONAL MODIFICATION

Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues.

SUBCELLULAR LOCATION

Golgi apparatus. Secreted, extracellular space, extracellular matrix. Note=Colocalizes with BMP1 in the Golgi.

FUNCTION

Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).