PRODUCT CODE: ET1704-65

Recombinant Periostin Monoclonal Antibody (ET1704-65)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

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Western blot analysis of Periostin on human placenta tissue lysate using anti-Periostin antibody at 1/1,000 dilution.
  • Western blot analysis of Periostin on human placenta tissue lysate using anti-Periostin antibody at 1/1,000 dilution.
  • Flow cytometric analysis of 293T cells with Periostin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Periostin on human placenta tissue lysate using anti-Periostin antibody at 1/1,000 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Periostin Monoclonal Antibody (ET1704-65)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta tissue lysate, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA63-40

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

89 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Periostin

SYNONYMS

Fasciclin-I like antibody; MGC119510 antibody; MGC119511 antibody; OSF 2 antibody; OSF-2 antibody; OSF2 antibody; Osteoblast specific factor 2 (fasciclin I like) antibody; Osteoblast specific factor 2 antibody; Osteoblast specific factor antibody; Osteoblast-specific factor 2 antibody; PDLPOSTN antibody; Periodontal ligament specific periostin antibody; Periostin antibody; Periostin isoform thy2 antibody; Periostin isoform thy4 antibody; Periostin isoform thy6 antibody; Periostin isoform thy8 antibody; Periostin osteoblast specific factor antibody; PN antibody; POSTN antibody; POSTN_HUMAN antibody; RP11 412K4.1 antibody

TISSUE SPECIFICITY

Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.

POST-TRANSLATIONAL MODIFICATION

Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta and alpha(V)beta integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor β 1(TGF β 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.