PRODUCT CODE: ET1703-38

Recombinant Perilipin A Monoclonal Antibody (ET1703-38)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Perilipin A on human liver cells lysates using anti-Perilipin A antibody at 1/500 dilution.
  • Western blot analysis of Perilipin A on human liver cells lysates using anti-Perilipin A antibody at 1/500 dilution.
  • ICC staining Perilipin A in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Perilipin A in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Perilipin A in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Perilipin A on human liver cells lysates using anti-Perilipin A antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Perilipin A Monoclonal Antibody (ET1703-38)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, HepG2, Hela, human liver cells lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-96

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

56 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Perilipin-1

GENE NAME

PLIN1

SYNONYMS

PLIN1

SEQUENCE SIMILARITIES

Belongs to the perilipin family.

TISSUE SPECIFICITY

Detected in adipocytes from white adipose tissue (at protein level). Detected in visceral adipose tissue and mammary gland.

POST-TRANSLATIONAL MODIFICATION

Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet (By similarity).

SUBCELLULAR LOCATION

Endoplasmic reticulum. Lipid droplet. Note=Lipid droplet surface-associated.

FUNCTION

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.