PRODUCT CODE: ET1705-99

Recombinant PDX1 Monoclonal Antibody (ET1705-99)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

IF analysis of paraffin-embedded human pancreas tissue using anti-PDX1 antibody (green). The nuclear counter stain is DAPI (blue).
  • IF analysis of paraffin-embedded human pancreas tissue using anti-PDX1 antibody (green). The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PDX1 antibody. Counter stained with hematoxylin.
IF analysis of paraffin-embedded human pancreas tissue using anti-PDX1 antibody (green). The nuclear counter stain is DAPI (blue).

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PDX1 Monoclonal Antibody (ET1705-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM62-45

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:50-1:200

  • ICC/IF

  • 1:50-1:200

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

PDX1

SYNONYMS

Glucose sensitive factor antibody; Glucose-sensitive factor antibody; GSF antibody; IDX 1 antibody; IDX-1 antibody; IDX1 antibody; Insulin promoter factor 1 antibody; Insulin promoter factor 1 homeodomain transcription factor antibody; Insulin upstream factor 1 antibody; IPF 1 antibody; IPF-1 antibody; IPF1 antibody; Islet/duodenum homeobox 1 antibody; Islet/duodenum homeobox-1 antibody; IUF 1 antibody; IUF-1 antibody; IUF1 antibody; MODY4 antibody; Pancreas/duodenum homeobox 1 antibody; Pancreas/duodenum homeobox protein 1 antibody; pancreatic and duodenal homeobox P antibody; PDX 1 antibody; PDX-1 antibody; PDX1 antibody; PDX1_HUMAN antibody; Somatostatin transactivating factor 1 antibody; Somatostatin-transactivating factor 1 antibody; STF 1 antibody; STF-1 antibody; STF1 antibody

SEQUENCE SIMILARITIES

Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.

TISSUE SPECIFICITY

Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. Phosphorylated by HIPK2 on Ser-268 upon glucose accumulation. This phosphorylation mediates subnuclear localization shifting. Phosphorylation by PASK may lead to translocation into the cytosol (By similarity).

SUBCELLULAR LOCATION

Cytosol, Nucleus.

FUNCTION

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.