PRODUCT CODE: ET1612-27

Recombinant PDPK1 Monoclonal Antibody (ET1612-27)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: PC-12 <br />
 Lane 3: HL-60
  • Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: PC-12 <br />
 Lane 3: HL-60
  • ICC staining PDPK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDPK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.
Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: PC-12
Lane 3: HL-60

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PDPK1 Monoclonal Antibody (ET1612-27)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T cell lysate, PC-12 cell lysate, HL-60 cell lysate, Hela, MCF-7, SW480, human breast carcinoma tissue, mouse kidney tissue, human colon carcinoma tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD204-9

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

63/48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PDPK1

SYNONYMS

3 phosphoinositide dependent protein kinase 1 antibody; 3-phosphoinositide-dependent protein kinase 1 antibody; hPDK 1 antibody; hPDK1 antibody; MGC20087 antibody; MGC35290 antibody; OTTHUMP00000159109 antibody; OTTHUMP00000159110 antibody; OTTHUMP00000174525 antibody; PDK1 antibody; Pdpk1 antibody; PDPK1_HUMAN antibody; PDPK2 antibody; PDPK2P antibody; PkB kinase antibody; PkB kinase like gene 1 antibody; PkB like 1 antibody; PRO0461 antibody; Protein kinase antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.

TISSUE SPECIFICITY

Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Ser-241 in the activation loop is required for full activity. PDPK1 itself can autophosphorylate Ser-241, leading to its own activation. Autophosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2 (By similarity). Tyr-9 phosphorylation is critical for stabilization of both PDPK1 and the PDPK1/SRC complex via HSP90-mediated protection of PDPK1 degradation. Angiotensin II stimulates the tyrosine phosphorylation of PDPK1 in vascular smooth muscle in a calcium- and SRC-dependent manner. Phosphorylated on Tyr-9, Tyr-373 and Tyr-376 by INSR in response to insulin. Palmitate negatively regulates autophosphorylation at Ser-241 and palmitate-induced phosphorylation at Ser-529 and Ser-501 by PKC/PRKCQ negatively regulates its ability to phosphorylate PKB/AKT1. Phosphorylation at Thr-354 by MELK partially inhibits kinase activity, the inhibition is cooperatively enhanced by phosphorylation at Ser-394 and Ser-398 by MAP3K5.; Autophosphorylated; autophosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2.; Monoubiquitinated in the kinase domain, deubiquitinated by USP4.

SUBCELLULAR LOCATION

Cell membrane, Nucleus, Cytoplasm, focal adhesion.

FUNCTION

PDPK1 (3-phosphoinositide dependent protein kinase 1), also known as PDK1, PDPK2, PDPK2P or PRO0461, is 556 amino acid ubiquitously expressed protein that localizes to the cell membrane, cytoplasm and nucleus. Acting as a master kinase, PDPK1 phosphorylates and activates a subgroup of the AGC family of protein kinases. PDPK1 is involved in mediating signal transduction for controlling proliferation, survival, and growth of developing pancreatic beta cells, regulating Ca2+ uptake and Ca2+-activated K+ channels of mast cells, regulation of chemotaxis and motility of vascular endothelial cells, cardiac homeostasis, and thymocyte development. Belonging to the protein kinase superfamily, PDPK1 contains a PH domain, which play an essential role in homodimerization, localization and nuclear import of PDPK1, and a protein kinase domain. PDPK1 exists as five alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p13.3.

CITATIONS

  • Cai, Guodong et al.

    Mechanism and effects of Zearalenone on mouse T lymphocytes activation in vitro. | Ecotoxicology and Environmental Safety [2018]