PRODUCT CODE: ET1702-70

Recombinant PDI Monoclonal Antibody (ET1702-70)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PDI on different lysates using anti-PDI antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: NIH/3T3 <br />
Lane 2: Human liver
  • Western blot analysis of PDI on different lysates using anti-PDI antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: NIH/3T3 <br />
Lane 2: Human liver
  • Flow cytometric analysis of Hela cells with PDI antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of PDI on different lysates using anti-PDI antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: Human liver

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PDI Monoclonal Antibody (ET1702-70)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF97-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Protein disulfide-isomerase

GENE NAME

P4HB

SYNONYMS

PDIp, Pdia2

SEQUENCE SIMILARITIES

Belongs to the protein disulfide isomerase family.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein.

FUNCTION

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.