PRODUCT CODE: ET1702-75

Recombinant PDHA1 Monoclonal Antibody (ET1702-75)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PDHA1 on different lysates using anti-PDHA1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: A431 <br />
 Lane 3: Mouse heart
  • Western blot analysis of PDHA1 on different lysates using anti-PDHA1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: A431 <br />
 Lane 3: Mouse heart
  • ICC staining PDHA1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDHA1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDHA1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with PDHA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of PDHA1 on different lysates using anti-PDHA1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: A431
Lane 3: Mouse heart

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PDHA1 Monoclonal Antibody (ET1702-75)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat kidney tissue lysate, mouse stomach tissue lysate, 293T cell lysate, A431 cell lysate, mouse heart tissue lysate, Hela, HepG2, SH-SY5Y, human breast carcinoma tissue, human kidney tissue, mouse skeletal muscle tissue, human lung carcinoma tissue, mouse colon tissue, mouse stomach tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF996-0

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PDHA1

SYNONYMS

ODPA_HUMAN antibody; PDH antibody; PDHA antibody; PDHA1 antibody; PDHCE1A antibody; PDHE1 A type I antibody; PDHE1-A type I antibody; PHE1A antibody; Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody; Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody; Pyruvate Dehydrogenase E1 alpha antibody; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.; Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).

SUBCELLULAR LOCATION

Mitochondrion matrix.

FUNCTION

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial matrix enzyme complex that functions as the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The E1 enzyme of the PDH complex is made up of a heterotetramer of two α and two β subunits. The E1-α subunit (PDH-E1α) contains the E1 active site and plays a key role in the function of the PDH complex. The PDH complex is regulated by phosphorylation and dephosphorylation of PDH-E1α. The gene encoding for PDH-E1α maps to chromosome Xp22.12, and a 20bp deletion in the last exon of this gene is sufficient to cause PDH deficiency, which causes a broad range of symptoms including the development of seizures, mental retardation and spasticity, as well as intermittent episodes of lactic acidosis associated with cerebellar ataxia.