Recombinant Rabbit monoclonal primary
Recombinant PDGF Receptor beta Monoclonal Antibody (ET1605-20)
NIH/3T3, human lung tissue, human spleen tissue, mouse lung tissue, mouse brain tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
PDGF Receptor beta
Beta platelet derived growth factor receptor antibody; Beta-type platelet-derived growth factor receptor antibody; CD 140B antibody; CD140 antigen-like family member B antibody; CD140b antibody; CD140b antigen antibody; IBGC4 antibody; IMF1 antibody; JTK12 antibody; OTTHUMP00000160528 antibody; PDGF R beta antibody; PDGF-R-beta antibody; PDGFR 1 antibody; PDGFR antibody; PDGFR beta antibody; PDGFR1 antibody; PDGFRB antibody; PGFRB_HUMAN antibody; Platelet derived growth factor receptor 1 antibody; Platelet derived growth factor receptor beta antibody; Platelet derived growth factor receptor beta polypeptide antibody
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-579, and to a lesser degree, at Tyr-581, is important for interaction with SRC family kinases. Phosphorylation at Tyr-740 and Tyr-751 is important for interaction with PIK3R1. Phosphorylation at Tyr-751 is important for interaction with NCK1. Phosphorylation at Tyr-771 and Tyr-857 is important for interaction with RASA1/GAP. Phosphorylation at Tyr-857 is important for efficient phosphorylation of PLCG1 and PTPN11, resulting in increased phosphorylation of AKT1, MAPK1/ERK2 and/or MAPK3/ERK1, PDCD6IP/ALIX and STAM, and in increased cell proliferation. Phosphorylation at Tyr-1009 is important for interaction with PTPN11. Phosphorylation at Tyr-1009 and Tyr-1021 is important for interaction with PLCG1. Phosphorylation at Tyr-1021 is important for interaction with CBL; PLCG1 and CBL compete for the same binding site. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. Dephosphorylated by PTPN2 at Tyr-579 and Tyr-1021.; N-glycosylated.; Ubiquitinated. After autophosphorylation, the receptor is polyubiquitinated, leading to its degradation.
Cell membrane, Cytoplasmic vesicle, Lysosome lumen.
Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and –β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two α receptors, PDGF-AB induces dimerization of αα and αβ and PDGF-BB induces the formation of three types of dimers, αα, αβ and ββ. Translocation of the PDGFR-∫ gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.