PRODUCT CODE: ET1704-70

Recombinant PDCD4 Monoclonal Antibody (ET1704-70)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

ICC staining PDCD4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDCD4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDCD4 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PDCD4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-PDCD4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PDCD4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PDCD4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with PDCD4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
ICC staining PDCD4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PDCD4 Monoclonal Antibody (ET1704-70)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, PC-3M, Hmuan tonsil tissue, human prostate tissue, human breasr cancer tissue, mouse colon tissue, MCF-7.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA80-83

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:20-1:50 IHC-P

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PDCD4

SYNONYMS

Death up-regulated gene protein antibody; Dug antibody; H731 antibody; Ma3 antibody; MGC33046 antibody; MGC33047 antibody; Neoplastic transformation inhibitor antibody; Neoplastic transformation inhibitor protein antibody; Nuclear antigen H731 antibody; Nuclear antigen H731 like antibody; Nuclear antigen H731 like protein antibody; Nuclear antigen H731-like antibody; PDCD 4 antibody; Pdcd4 antibody; PDCD4_HUMAN antibody; Programmed cell death 4 antibody; programmed cell death 4 (neoplastic transformation inhibitor) antibody; Programmed cell death protein 4 antibody; Protein 197/15a antibody; Protein MA-3 antibody; Tis antibody; Topoisomerase-inhibitor suppressed protein antibody

SEQUENCE SIMILARITIES

Belongs to the PDCD4 family.

TISSUE SPECIFICITY

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.; Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

The transformation suppressor gene Pdcd-4 (programmed cell death gene 4) inhibits the tumor-promoter mediated transformation of mouse keratinocytes and is a potential tumor suppressor gene in the development of human lung cancer. Biochemical analysis suggests that the Pdcd-4 protein is involved in protein translation as well as in nuclear events. Pdcd-4 directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Pdcd-4 also suppresses the transactivation of AP-1 responsive promoters by c-Jun, suggesting that the transformation-suppressor activity of Pdcd-4 might be due, at least in part, to the inhibition of c-Jun activity. In addition to affecting c-Jun phosphorylation, Pdcd-4 blocks the recruitment of the co-activator p300 by c-Jun.