PRODUCT CODE: ET1701-41

Recombinant PD-L1 Monoclonal Antibody (ET1701-41)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PD-L1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: A549 cell lysates<br />
Lane 2: MCF-7 cell lysates<br />
Lane 3: Mouse placenta tissue lysates<br />
Lane 4: Mouse lung tissue lysates
  • Western blot analysis of PD-L1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: A549 cell lysates<br />
Lane 2: MCF-7 cell lysates<br />
Lane 3: Mouse placenta tissue lysates<br />
Lane 4: Mouse lung tissue lysates
  • Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer tissue using anti-PD-L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of PD-L1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysates
Lane 2: MCF-7 cell lysates
Lane 3: Mouse placenta tissue lysates
Lane 4: Mouse lung tissue lysates

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PD-L1 Monoclonal Antibody (ET1701-41)

Immunogen

Synthetic peptide within human pd-l1 aa 200-230 (extracellular).

Host

Rabbit

Positive Control

A549 cell lysate, MCF-7 cell lysate, mouse placenta tissue lysate, mouse lung tissue lysate, human non-small cell lung cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ08-95

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

33 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Programmed cell death 1 ligand 1

GENE NAME

CD274

SYNONYMS

PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7-H1, CD274

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. BTN/MOG family.

TISSUE SPECIFICITY

Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein; Single-pass type I membrane protein; Single-pass type I membrane protein.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein; Single-pass type I membrane protein.

FUNCTION

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10).; The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).