PRODUCT CODE: ET7106-79

Recombinant PCAF Monoclonal Antibody (ET7106-79)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PCAF on rat kidney tissue (1) and K562 cell (2) lysate using anti-PCAF antibody at 1/500 dilution.
  • Western blot analysis of PCAF on rat kidney tissue (1) and K562 cell (2) lysate using anti-PCAF antibody at 1/500 dilution.
  • Flow cytometric analysis of Hela cells with PCAF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of PCAF on rat kidney tissue (1) and K562 cell (2) lysate using anti-PCAF antibody at 1/500 dilution.

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PCAF Monoclonal Antibody (ET7106-79)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat kidney tissue lysate, K562, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU88-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

93 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

PCAF

SYNONYMS

CAF antibody; CREBBP associated factor antibody; GCN5 antibody; GCN5L antibody; GCN5L1 antibody; Histone acetylase PCAF antibody; Histone acetyltransferase KAT2B antibody; Histone acetyltransferase PCAF antibody; K(lysine) acetyltransferase 2B antibody; KAT2B antibody; KAT2B_HUMAN antibody; Lysine acetyltransferase 2B antibody; P antibody; P/CAF antibody; p300/CBP associated factor antibody; P300/CBP-associated factor antibody; Pcaf antibody

SEQUENCE SIMILARITIES

Belongs to the acetyltransferase family. GCN5 subfamily.

TISSUE SPECIFICITY

Ubiquitously expressed but most abundant in heart and skeletal muscle. Also expressed in the skin, in keratinocytes (at protein level).

DEVELOPMENTAL STAGE

Up-regulated during keratinocyte differentiation (at protein level).

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessiblity to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.