PRODUCT CODE: ET1612-60

Recombinant PAX7 Monoclonal Antibody (ET1612-60)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PAX7 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of PAX7 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of PAX7 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-60, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of PAX7 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PAX7 Monoclonal Antibody (ET1612-60)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysates, MCF-7.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD083-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

57 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PAX7

SYNONYMS

FLJ37460 antibody; HUP1 antibody; OTTHUMP00000002534 antibody; Paired box 7 antibody; Paired box gene 7 antibody; Paired box homeotic gene 7 antibody; Paired box protein Pax-7 antibody; Paired domain gene 7 antibody; Paired domain gene HuP1 antibody; Pax7 antibody; PAX7 transcriptional factor antibody; PAX7/FKHR fusion gene, included antibody; PAX7_HUMAN antibody; PAX7B antibody; RGD1564360 antibody; RMS2 antibody

SEQUENCE SIMILARITIES

Belongs to the paired homeobox family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).