Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant PAX5 Monoclonal Antibody (ET1701-49)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Daudi cell lysates, Hela, MCF-7, HepG2, human spleen tissue, human tonsil tissue, Raji.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ08-87
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
42 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
PAX5
SYNONYMS
B cell lineage specific activator antibody; B cell lineage specific activator protein antibody; B cell specific activator protein antibody; B cell specific transcription factor antibody; B-cell-specific transcription factor antibody; BSAP antibody; EBB-1 antibody; KLP antibody; Paired box 5 antibody; Paired box gene 5 (B cell lineage specific activator protein) antibody; Paired box gene 5 (B cell lineage specific activator) antibody; Paired box gene 5 antibody; Paired box homeotic gene 5 antibody; Paired box protein Pax 5 antibody; Paired box protein Pax-5 antibody; Paired domain gene 5 antibody; PAX 5 antibody; PAX5 antibody; PAX5_HUMAN antibody; Transcription factor PAX 5 antibody
DEVELOPMENTAL STAGE
Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
POST-TRANSLATIONAL MODIFICATION
O-glycosylated.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The Pax family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. Pax family genes are also involved in a variety of signal transduction pathways in the adult organism. Mutations in Pax proteins have been linked to disease and cancer in humans. For example, the human PAX5 gene encodes a B cell lineage-specific protein, Pax-5, also designated B cell specific activator protein or BSAP, which is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. Pax-5 functions to regulate not only B cell development, but also influences the balance between immunoglobulin secretion and B cell proliferation. Overexpression of Pax-5 has been implicated in cellular transformation, and in the case of small lymphocytic lymphomas with plasmacytoid differentiation, a t(9;14)(p13;q32) translocation resulting in the deregulation of PAX5 gene expression has been detected.