PRODUCT CODE: ET1610-26

Recombinant p95 NBS1 Monoclonal Antibody (ET1610-26)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

  • IHC-P

  • CHIP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Nibrin on human lung lysates using anti-p95 NBS1 antibody at 1/1,000 dilution.
  • Western blot analysis of Nibrin on human lung lysates using anti-p95 NBS1 antibody at 1/1,000 dilution.
  • ICC staining p95 NBS1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining p95 NBS1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Hela cells with p95 NBS1antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Nibrin on human lung lysates using anti-p95 NBS1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

  • IHC-P

  • CHIP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant p95 NBS1 Monoclonal Antibody (ET1610-26)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, Hela, human lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC05-68

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

95 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IHC-P:1:50

TARGET

UNIPROT #

PROTEIN NAME

Nibrin

GENE NAME

NBN

SYNONYMS

NBN

TISSUE SPECIFICITY

Ubiquitous. Expressed at high levels in testis.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.

SUBCELLULAR LOCATION

Nucleus. Nucleus, PML body. Chromosome, telomere. Chromosome. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents.

FUNCTION

Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.