PRODUCT CODE: ET1609-80

Recombinant p73 Monoclonal Antibody (ET1609-80)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of p73 on Hela cell lysates using anti-p73 antibody at 1/1,000 dilution.
  • Western blot analysis of p73 on Hela cell lysates using anti-p73 antibody at 1/1,000 dilution.
  • ICC staining p73 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p73 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-p73 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-p73 antibody. Counter stained with hematoxylin.
Western blot analysis of p73 on Hela cell lysates using anti-p73 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant p73 Monoclonal Antibody (ET1609-80)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293 cell lysates, MCF-7, human kidney tissue, mouse skin tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST05-76

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

70/60 kDa(12 isoforms)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

p73

SYNONYMS

p53 like transcription factor antibody; p53 related protein antibody; p53-like transcription factor antibody; p53-related protein antibody; p73 antibody; P73_HUMAN antibody; TP73 antibody; Tumor protein p73 antibody

SEQUENCE SIMILARITIES

Belongs to the p53 family.

TISSUE SPECIFICITY

Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.

POST-TRANSLATIONAL MODIFICATION

Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function.; Higher levels of phosphorylation seen in the brain from patients with Huntington disease.; Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p53 shares considerable sequence similarity with p73, a gene that maps to a region in chromosome 1 that is frequently deleted in neuroblastomas. However, p73 does not appear to be activated by DNA damaging agents. The p73 isoform p73α inhibits drug-induced apoptosis in small cell lung carcinoma cells, while the p73 isoform p73β promotes it. p73α also prevents Bax activation, mitochondrial dysfunction, caspase activation and is able to reduce apoptosis induced by the BH3-only protein PUMA (p53 upregulated modulator of apoptosis). There is an equilibrium between p73α and p73β, demonstrated by the fact that p73α inhibits the pro-apoptotic effect of p73β.