PRODUCT CODE: ET1602-26

Recombinant p38 Monoclonal Antibody (ET1602-26)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of p38 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: Hela cell lysate<br />
 Lane 2: NIH/3T3 cell lysate<br />
 Lane 3: PC-12 cell lysate<br />
 Lane 4: Jurkat cell lysate
  • Western blot analysis of p38 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: Hela cell lysate<br />
 Lane 2: NIH/3T3 cell lysate<br />
 Lane 3: PC-12 cell lysate<br />
 Lane 4: Jurkat cell lysate
  • ICC staining of p38 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of p38 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of p38 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Jurkat cell lysate

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant p38 Monoclonal Antibody (ET1602-26)

Immunogen

Synthetic peptide within human p38 aa 160-200.

Host

Rabbit

Positive Control

Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Jurkat cell lysate, Hela, NIH/3T3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR43-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

p38

SYNONYMS

CSAID Binding Protein 1 antibody; CSAID binding protein antibody; CSAID-binding protein antibody; Csaids binding protein antibody; CSBP 1 antibody; CSBP 2 antibody; CSBP antibody; CSBP1 antibody; CSBP2 antibody; CSPB1 antibody; Cytokine suppressive anti-inflammatory drug-binding protein antibody; EXIP antibody; MAP kinase 14 antibody; MAP kinase MXI2 antibody; MAP kinase p38 alpha antibody; MAPK 14 antibody; MAPK14 antibody; MAX interacting protein 2 antibody; MAX-interacting protein 2 antibody; Mitogen Activated Protein Kinase 14 antibody; Mitogen activated protein kinase p38 alpha antibody; Mitogen-activated protein kinase 14 antibody; Mitogen-activated protein kinase p38 alpha antibody; MK14_HUMAN antibody; Mxi 2 antibody; MXI2 antibody; p38 ALPHA antibody; p38 antibody; p38 MAP kinase antibody; p38 MAPK antibody; p38 mitogen activated protein kinase antibody; p38ALPHA antibody; p38alpha Exip antibody; PRKM14 antibody; PRKM15 antibody; RK antibody; SAPK2A antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

TISSUE SPECIFICITY

Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.

POST-TRANSLATIONAL MODIFICATION

Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16. PPM1D also mediates dephosphorylation and inactivation of MAPK14.; Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3.; Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein.

CITATIONS

  • Chen, Qiuhua et al.

    Sini decoction ameliorates sepsis-induced acute lung injury via regulating ACE2-Ang (1-7)-Mas axis and inhibiting the MAPK signaling pathway. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2019]