PRODUCT CODE: ET1706-43

Recombinant p23 Monoclonal Antibody (ET1706-43)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of p23 on different lysates using anti-p23 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Mouse brain tissue<br />
 Lane 2: SK-Br-3<br />
 Lane 3: Rat lung tissue<br />
  • Western blot analysis of p23 on different lysates using anti-p23 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Mouse brain tissue<br />
 Lane 2: SK-Br-3<br />
 Lane 3: Rat lung tissue<br />
  • ICC staining p23 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining p23 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p23 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p23 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-p23 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-p23 antibody. Counter stained with hematoxylin.
Western blot analysis of p23 on different lysates using anti-p23 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain tissue
Lane 2: SK-Br-3
Lane 3: Rat lung tissue

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant p23 Monoclonal Antibody (ET1706-43)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse brain tissue lysate, SK-Br-3, rat lung tissue lysate, A431, Hela, human tonsil tissue, human colon cancer tissue, mouse fallopian tube tissue, rat testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU09-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Prostaglandin E synthase 3

GENE NAME

PTGES3

SYNONYMS

cPGES, PTGES3, P23, TEBP

SEQUENCE SIMILARITIES

Belongs to the p23/wos2 family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway.