PRODUCT CODE: ET1701-76

Recombinant Occludin Monoclonal Antibody (ET1701-76)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Flow cytometric analysis of 293 cells with Occludin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
  • Flow cytometric analysis of 293 cells with Occludin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Flow cytometric analysis of 293 cells with Occludin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Occludin Monoclonal Antibody (ET1701-76)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ091-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Occludin

GENE NAME

OCLN

SYNONYMS

OCLN

SEQUENCE SIMILARITIES

Belongs to the ELL/occludin family.

TISSUE SPECIFICITY

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

POST-TRANSLATIONAL MODIFICATION

Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein.

FUNCTION

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.