PRODUCT CODE: ET1706-52

Recombinant Nuclear Matrix Protein p84 Monoclonal Antibody (ET1706-52)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Nuclear Matrix Protein p84 on different lysates using anti-Nuclear Matrix Protein p84 antibody at 1/500 dilution.<br />
 Positive control:<br />
  Lane 1: Hela<br />
  Lane 2: A431<br />
  Lane 3: Mouse skeletal muscle<br />
  Lane 4: PC-12
  • Western blot analysis of Nuclear Matrix Protein p84 on different lysates using anti-Nuclear Matrix Protein p84 antibody at 1/500 dilution.<br />
 Positive control:<br />
  Lane 1: Hela<br />
  Lane 2: A431<br />
  Lane 3: Mouse skeletal muscle<br />
  Lane 4: PC-12
  • ICC staining Nuclear Matrix Protein p84 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Nuclear Matrix Protein p84 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Nuclear Matrix Protein p84 antibody. Counter stained with hematoxylin.
Western blot analysis of Nuclear Matrix Protein p84 on different lysates using anti-Nuclear Matrix Protein p84 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: A431
Lane 3: Mouse skeletal muscle
Lane 4: PC-12

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Nuclear Matrix Protein p84 Monoclonal Antibody (ET1706-52)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, A431, mouse skeletal muscle tissue lysate, PC-12, HUVEC, LOVO, rat testis tissue, rat brain tissue, human tonsil tissue, human lung cancer tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU53-18

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

76 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Nuclear Matrix Protein p84

SYNONYMS

hTREX84 antibody; Death domain containing protein p84N5 antibody; HPR 1 antibody; HPR1 antibody; hTREX84 antibody; Nuclear matrix protein p84 antibody; P84 antibody; p84N5 antibody; Tho 1 antibody; THO complex 1 antibody; THO complex subunit 1 antibody; Tho1 antibody; THOC 1 antibody; Thoc1 antibody; THOC1_HUMAN antibody

TISSUE SPECIFICITY

Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.

POST-TRANSLATIONAL MODIFICATION

Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.; Polyubiquitinated, leading to proteasomal degradation; probably involves NEDD4.

SUBCELLULAR LOCATION

SwissProt: Q96FV9(Human) Q8R3N6(Mouse) P59924(Rat)

FUNCTION

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy.