PRODUCT CODE: ET1611-39

Recombinant NSD3 Monoclonal Antibody (ET1611-39)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: MCF-7 <br />
 Lane 2: 293T
  • Western blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: MCF-7 <br />
 Lane 2: 293T
  • Flow cytometric analysis of Hela cells with NSD3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: 293T

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NSD3 Monoclonal Antibody (ET1611-39)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN07-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

162 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NSD3

SYNONYMS

DKFZp667H044 antibody; FLJ20353 antibody; Histone lysine N methyltransferase NSD3 antibody; Histone-lysine N-methyltransferase NSD3 antibody; MGC126766 antibody; MGC142029 antibody; NSD 3 antibody; NSD3_HUMAN antibody; Nuclear set domain containing 3 antibody; Nuclear SET domain containing protein 3 antibody; Nuclear SET domain-containing protein 3 antibody; pp14328 antibody; Protein whistle antibody; Whistle antibody; WHSC1 like protein 1 antibody; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody; WHSC1-like protein 1 antibody; WHSC1L1 antibody; WHSC1L1 protein antibody; Wolf Hirschhorn syndrome candidate 1 like 1 antibody; Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody

SEQUENCE SIMILARITIES

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.

TISSUE SPECIFICITY

Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.

SUBCELLULAR LOCATION

Nucleus, Chromosome.

FUNCTION

The deduced 1,437 amino acid NSD3 protein contains two PWWP domains involved in protein-protein interactions, five PHD-type zinc finger motifs found in chromatin-associated proteins, a SAC (SET-associated cys-rich) domain, a SET domain and a C-terminal C5HCH domain. Two NSD3 variants have been identified. The short variant comprised of 645 amino acids, arises from alternative polyadenylation and exon splicing and contains a single PWWP domain. A longer NSD3 variant, which is only expressed in HeLa cells, is comprised of 1,388 amino acid residues. The human WHSC1L1 gene, which encodes the NSD3 protein, shares 68% and 55% identity with mouse Nsd1 and human WHSC1, respectively. Highest expression of NSD3 is observed in brain, heart and skeletal muscle tissues; lower levels of NSD3 expression are observed in the liver and lungs.