PRODUCT CODE: ET1705-86

Recombinant NRF1 Monoclonal Antibody (ET1705-86)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NRF1 on Hela cell (1) and mouse heart tissue (2) lysate using anti-NRF1 antibody at 1/500 dilution.
  • Western blot analysis of NRF1 on Hela cell (1) and mouse heart tissue (2) lysate using anti-NRF1 antibody at 1/500 dilution.
  • ICC staining NRF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NRF1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NRF1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NRF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NRF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyriod tissue using anti-NRF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NRF1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with NRF1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of NRF1 on Hela cell (1) and mouse heart tissue (2) lysate using anti-NRF1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NRF1 Monoclonal Antibody (ET1705-86)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysates, Hela, MCF-7, SH-SY5Y, rat brain tissue, human tonsil tissue, human thyroid tissue, mouse colon tissue, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM89-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

56 kDa/65kDa(Observed)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NRF1

SYNONYMS

alpha pal antibody; alpha palindromic binding protein antibody; Alpha palindromic-binding protein antibody; Alpha-pal antibody; locus control region factor 1 antibody; NFE2 related factor 1 antibody; NRF-1 antibody; Nrf1 antibody; NRF1_HUMAN antibody; Nuclear respiratory factor 1 antibody

SEQUENCE SIMILARITIES

Belongs to the NRF1/Ewg family.

TISSUE SPECIFICITY

Ubiquitously expressed with strongest expression in skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation enhances DNA binding.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.