PRODUCT CODE: ET7106-85

Recombinant NR0B1 Monoclonal Antibody (ET7106-85)

  • Recombinant

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of NR0B1 on A549 cell lysate using anti-NR0B1 antibody at 1/500 dilution.
  • Western blot analysis of NR0B1 on A549 cell lysate using anti-NR0B1 antibody at 1/500 dilution.
  • ICC staining NR0B1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NR0B1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NR0B1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of A549 cells with NR0B1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of NR0B1 on A549 cell lysate using anti-NR0B1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NR0B1 Monoclonal Antibody (ET7106-85)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, Hela, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU33-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Nuclear receptor subfamily 0 group B member 1

GENE NAME

NR0B1

SYNONYMS

NR0B1, AHC, DAX1

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR0 subfamily.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Note=Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus.

FUNCTION

Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).