PRODUCT CODE: ET1702-50

Recombinant NQO1 Monoclonal Antibody (ET1702-50)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: SH-SY-5Y <br />
Lane 2: Mouse kidney <br />
Lane 3: Raji
  • Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: SH-SY-5Y <br />
Lane 2: Mouse kidney <br />
Lane 3: Raji
  • Flow cytometric analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY-5Y
Lane 2: Mouse kidney
Lane 3: Raji

Applications

  • WB

  • ICC

  • IF

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NQO1 Monoclonal Antibody (ET1702-50)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Raji, SH-SY-5Y, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF440-1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

31/27 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:50-1:200

  • IP

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

NAD(P)H dehydrogenase [quinone] 1

GENE NAME

NQO1

SYNONYMS

DTD, QR1, NQO1

SEQUENCE SIMILARITIES

Belongs to the NAD(P)H dehydrogenase (quinone) family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

CITATIONS

  • Liu, Suzi et al.

    Sinomenine protects against E.coli-induced acute lung injury in mice through Nrf2-NF-�B pathway. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2018]