UNIPROT #

P15559

PROTEIN NAME

NQO1

SYNONYMS

Azoreductase antibody; Cytochrome b 5 reductase antibody; DHQU antibody; DIA 4 antibody; DIA4 antibody; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody; Diaphorase (NADH/NADPH) antibody; Diaphorase 4 antibody; Dioxin inducible 1 antibody; DT diaphorase antibody; DT-diaphorase antibody; DTD antibody; Menadione reductase antibody; NAD(P)H dehydrogenase [quinone] 1 antibody; NAD(P)H dehydrogenase quinone 1 antibody; NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody; NAD(P)H:menadione oxidoreductase 1 antibody; NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody; NAD(P)H:quinone oxidoreductase 1 antibody; NAD(P)H:quinone oxireductase antibody; NMOR 1 antibody; NMOR I antibody; NMOR1 antibody; NMORI antibody; NQO 1 antibody; NQO1 antibody; NQO1_HUMAN antibody; Phylloquinone reductase antibody; QR 1 antibody; QR1 antibody; Quinone reductase 1 antibody

SEQUENCE SIMILARITIES

Belongs to the NAD(P)H dehydrogenase (quinone) family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.