Positive control:
Lane 1: SH-SY-5Y
Lane 2: Mouse kidney
Lane 3: Raji
Applications
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WB
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ICC
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IF
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IP
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FC
REACTIVITY
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Human
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Mouse
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Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant NQO1 Monoclonal Antibody (ET1702-50)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Mouse kidney tissue lysate, SH-SY5Y cell lysate, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF440-1
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
31/27 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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FC
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1:50-1:100
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ICC/IF
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1:50-1:200
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IP
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1:50
TARGET
UNIPROT #
PROTEIN NAME
NQO1
SYNONYMS
Azoreductase antibody; Cytochrome b 5 reductase antibody; DHQU antibody; DIA 4 antibody; DIA4 antibody; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody; Diaphorase (NADH/NADPH) antibody; Diaphorase 4 antibody; Dioxin inducible 1 antibody; DT diaphorase antibody; DT-diaphorase antibody; DTD antibody; Menadione reductase antibody; NAD(P)H dehydrogenase [quinone] 1 antibody; NAD(P)H dehydrogenase quinone 1 antibody; NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody; NAD(P)H:menadione oxidoreductase 1 antibody; NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody; NAD(P)H:quinone oxidoreductase 1 antibody; NAD(P)H:quinone oxireductase antibody; NMOR 1 antibody; NMOR I antibody; NMOR1 antibody; NMORI antibody; NQO 1 antibody; NQO1 antibody; NQO1_HUMAN antibody; Phylloquinone reductase antibody; QR 1 antibody; QR1 antibody; Quinone reductase 1 antibody
SEQUENCE SIMILARITIES
Belongs to the NAD(P)H dehydrogenase (quinone) family.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
CITATIONS
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Liu, Suzi et al.
Sinomenine protects against E.coli-induced acute lung injury in mice through Nrf2-NF-�B pathway. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2018]
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WB
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IHC
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mouse
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Citation
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