PRODUCT CODE: ET1704-35

Recombinant Noxa Monoclonal Antibody (ET1704-35)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Noxa antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Noxa antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noxa antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with Noxa antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Noxa antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Noxa Monoclonal Antibody (ET1704-35)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human liver tissue, human spleen tissue, human kidney tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA30-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

6 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

Noxa

SYNONYMS

Adult T cell leukemia derived PMA responsive antibody; APR antibody; APR_HUMAN antibody; ATL-derived antibody; Immediate early response protein APR antibody; Immediate-early-response protein APR antibody; NOXA antibody; Phorbol 12 myristate 13 acetate induced protein 1 antibody; Phorbol-12-myristate-13-acetate-induced protein 1 antibody; PMA induced protein 1 antibody; PMA-induced protein 1 antibody; PMA-responsive gene antibody; Pmaip1 antibody; Protein Noxa antibody

SEQUENCE SIMILARITIES

Belongs to the PMAIP1 family.

TISSUE SPECIFICITY

Highly expressed in adult T-cell leukemia cell line.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

Members of the Bcl-2 family of proteins interact to regulate programmed cell death (apoptosis) under a broad range of physiological conditions. Bcl-2, Bcl-xL, and several related proteins inhibit apoptosis, whereas other members of the Bcl-2 family, such as Bax and Bak, enhance cell death. NOXA, a pro-apoptotic member of the Bcl-2 family, contains the Bcl-2 homology 3 (BH3) region, but does not contain other BH domains. Murine cells constitutively express NOXA mRNA in small amounts in various organs; X-ray irradiation increases NOXA mRNA and protein expression levels. In human cells, NOXA, alternatively designated PMA-induced protein 1 or APR, displays high expression in the adult T cell leukemia cell line IKD, where it may function as an immediate-early-response gene. The NOXA protein selectively localizes to mitochondria.