PRODUCT CODE: ET1704-80

Recombinant NMDAR2A Monoclonal Antibody (ET1704-80)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NMDAR2A on rat brain tissue lysate using anti-NMDAR2A antibody at 1/1,000 dilution.
  • Western blot analysis of NMDAR2A on rat brain tissue lysate using anti-NMDAR2A antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat skin tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-NMDAR2A antibody. Counter stained with hematoxylin.
Western blot analysis of NMDAR2A on rat brain tissue lysate using anti-NMDAR2A antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NMDAR2A Monoclonal Antibody (ET1704-80)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue lysate, rat brain tissue, rat skin tissue, human brain tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA31-20

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

165 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Glutamate receptor ionotropic, NMDA 2A

GENE NAME

GRIN2A

SYNONYMS

GluN2A, NMDAR2A, NR2A, hNR2A, GRIN2A, NMDAR2A

SEQUENCE SIMILARITIES

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.

SUBCELLULAR LOCATION

Cell projection, dendritic spine; Multi-pass membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane. Note=Expression at the dendrite cell membrane and at synapses is regulated by SORCS2 and the retromer complex.

FUNCTION

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have lower sensitivity to glutamate and faster deactivation kinetics than channels formed by GRIN1 and GRIN2B. Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity).