PRODUCT CODE: ET1702-78

Recombinant Neutrophil Elastase Monoclonal Antibody (ET1702-78)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

ICC staining Neutrophil Elastase in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Neutrophil Elastase in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Neutrophil Elastase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Neutrophil Elastase in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Neutrophil Elastase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Neutrophil Elastase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Neutrophil Elastase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
ICC staining Neutrophil Elastase in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Neutrophil Elastase Monoclonal Antibody (ET1702-78)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse spleen tissue lysate, mouse bone marrow tissue lysate, A549, Hela, HepG2, human bone marrow tissue, mouse bone tissue, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF098-6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

29 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50 IHC-P

  • FC

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Neutrophil Elastase

SYNONYMS

Bone marrow serine protease antibody; ELA2 antibody; ELANE antibody; Elastase 2 antibody; Elastase 2 neutrophil antibody; Elastase neutrophil expressed antibody; Elastase-2 antibody; ELNE_HUMAN antibody; GE antibody; Granulocyte derived elastase antibody; HLE antibody; HNE antibody; Human leukocyte elastase antibody; Leukocyte elastase antibody; Medullasin antibody; NE antibody; Neutrophil elastase antibody; PMN E antibody; PMN elastase antibody; Polymorphonuclear elastase antibody; SCN1 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase S1 family. Elastase subfamily.

TISSUE SPECIFICITY

Bone marrow cells. Neutrophil.

SUBCELLULAR LOCATION

Phagosome.

FUNCTION

Neutrophil Elastase (NE) is a serine protease that is expressed in bone marrow precursor cells, stored in peripheral blood granulocytes, and implicated in the progression of a variety of inflammatory diseases, including idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome and cystic fibrosis. In neutrophils, Neutrophil Elastase contributes largely to the proteolysis of phagocytosed proteins, the migration of neutrophils and the remodeling of tissues following injury. Neutrophil Elastase, which is also designated medullasin, is secreted into the extracellular matrix, where it is then capable of destroying connective tissue proteins, including elastin, proteoglycans and Type IV Collagens. Neutrophil Elastase also mediates proteolysis by cleaving proteins that are associated with the complement system, such as antithrombin and Fibrinogen. Additionally, Neutrophil Elastase functions as a potent platelet agonist, where it potentiates the aggregation, secretion and mobilization of calcium in response to cathepsin G binding to platelet surface receptors.