PRODUCT CODE: ET1702-78

Recombinant Neutrophil Elastase Monoclonal Antibody (ET1702-78)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

ICC staining Neutrophil Elastase in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Neutrophil Elastase in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Neutrophil Elastase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Neutrophil Elastase in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Neutrophil Elastase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Neutrophil Elastase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Neutrophil Elastase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
ICC staining Neutrophil Elastase in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Neutrophil Elastase Monoclonal Antibody (ET1702-78)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, Hela, HepG2, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF098-6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

29 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50 IHC-P

  • FC

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Neutrophil elastase

GENE NAME

ELANE

SYNONYMS

HLE, ELANE

SEQUENCE SIMILARITIES

Belongs to the peptidase S1 family. Elastase subfamily.

TISSUE SPECIFICITY

Bone marrow cells. Neutrophil.

SUBCELLULAR LOCATION

Cytoplasmic vesicle, phagosome. Note=Localized in phagolysosomes following ingestion of E.coli by neutrophils.

FUNCTION

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae.