PRODUCT CODE: ET1703-73

Recombinant NeuroD1 Monoclonal Antibody (ET1703-73)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NeuroD1 on different cells lysates using anti-NeuroD1 antibody at 1/500 dilution.<br />
Positive control: <br />
Line 1: human brain <br />
Line 2:SH-SY5Y
  • Western blot analysis of NeuroD1 on different cells lysates using anti-NeuroD1 antibody at 1/500 dilution.<br />
Positive control: <br />
Line 1: human brain <br />
Line 2:SH-SY5Y
  • Western blot analysis of NeuroD1 on Li-7 cell lysates using anti-NeuroD1 antibody at 1/500 dilution.
Western blot analysis of NeuroD1 on different cells lysates using anti-NeuroD1 antibody at 1/500 dilution.
Positive control:
Line 1: human brain
Line 2:SH-SY5Y

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NeuroD1 Monoclonal Antibody (ET1703-73)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human brain, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Neurogenic differentiation factor 1

GENE NAME

NEUROD1

SYNONYMS

NeuroD, NeuroD1, bHLHa3, NEUROD1

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. Phosphorylated by MAPK1; phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells (By similarity).

SUBCELLULAR LOCATION

Cytoplasm. Note=In pancreatic islet cells, shuttles to the nucleus in response to glucose stimulation (By similarity). Colocalizes with NR0B2 in the nucleus.

FUNCTION

Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).