PRODUCT CODE: ET7107-28

Recombinant Nesprin 1 Monoclonal Antibody (ET7107-28)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Nesprin 1 on different lysates using anti-Nesprin 1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: A549 <br />
  Lane 2: Mouse spleen tissue
  • Western blot analysis of Nesprin 1 on different lysates using anti-Nesprin 1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: A549 <br />
  Lane 2: Mouse spleen tissue
  • ICC staining Nesprin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Nesprin 1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Nesprin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Daudi cells with Nesprin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Nesprin 1 on different lysates using anti-Nesprin 1 antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 2: Mouse spleen tissue

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Nesprin 1 Monoclonal Antibody (ET7107-28)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, mouse spleen tissue lysate, C2C12, HUVEC, rat brain tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse fallopian tubes tissue, Daudi.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB35-26

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

112 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:200-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Nesprin 1

SYNONYMS

8B antibody; ARCA1 antibody; C6orf98 antibody; CPG2 antibody; CPG2 full length antibody; dJ45H2.2 antibody; EDMD4 antibody; Enaptin antibody; Myne-1 antibody; MYNE1 antibody; Myocyte nuclear envelope protein 1 antibody; Nesp1 antibody; Nesprin-1 antibody; Nuclear envelope spectrin repeat protein 1 antibody; SCAR8 antibody; Spectrin repeat containing nuclear envelope 1 antibody; Synaptic nuclear envelope protein 1 antibody; Synaptic nuclei expressed gene 1 antibody; Syne-1 antibody; SYNE1 antibody; SYNE1_HUMAN antibody; SYNE1B antibody

SEQUENCE SIMILARITIES

Belongs to the nesprin family.

TISSUE SPECIFICITY

Expressed in HeLa, A431, A172 and HaCaT cells (at protein level). Widely expressed. Highly expressed in skeletal and smooth muscles, heart, spleen, peripheral blood leukocytes, pancreas, cerebellum, stomach, kidney and placenta. Isoform GSRP-56 is predominantly expressed in heart and skeletal muscle (at protein level).

POST-TRANSLATIONAL MODIFICATION

The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces.

SUBCELLULAR LOCATION

Cytoskeleton. Nucleus.

FUNCTION

Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes; SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.