PRODUCT CODE: ET1703-57

Recombinant NEFM Monoclonal Antibody (ET1703-57)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.
  • Western blot analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.
  • ICC staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NEFM in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NEFM in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.
Western blot analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NEFM Monoclonal Antibody (ET1703-57)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, N2A, PC-12, SH-SY5Y, mouse brain tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-20

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

160 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Neurofilament medium polypeptide

GENE NAME

NEFM

SYNONYMS

NF-M, NEFM

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

POST-TRANSLATIONAL MODIFICATION

There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.; Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.; Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton. Cell projection, axon.

FUNCTION

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).