PRODUCT CODE: ET1611-42

Recombinant NEDD4-2 Monoclonal Antibody (ET1611-42)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NEDD4-2 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of NEDD4-2 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NEDD4-2 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NEDD4-2 Monoclonal Antibody (ET1611-42)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat cell lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN73-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

112 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

TARGET

UNIPROT #

PROTEIN NAME

NEDD4-2

SYNONYMS

E3 ubiquitin protein ligase NEDD4 like protein antibody; E3 ubiquitin-protein ligase NEDD4-like antibody; KIAA0439 antibody; NED4L_HUMAN antibody; NEDD4 2 antibody; NEDD4 2c antibody; Nedd4-2 antibody; NEDD4-2a antibody; NEDD4.2 antibody; NEDD4b antibody; NEDD4L antibody; NEDD4La antibody; NEDD4Lb antibody; NEDD4Lc antibody; NEDD4Ld antibody; NEDD4Le antibody; NEDD4Lf antibody; NEDD4Lg antibody; NEDD4Lh antibody; NEDL3 antibody; Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody; Neural precursor cell expressed, developmentally down regulated 4 like antibody; RSP5 antibody; Ubiquitin protein ligase Rsp5 antibody

TISSUE SPECIFICITY

Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Expressed in melanocytes.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.; Auto-ubiquitinated. Deubiquitinated by USP36, no effect on NEDD4L protein levels. Both proteins interact and regulate each other's ubiquitination levels.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

NEDD4-L (neural precursor cell expressed, developmentally down-regulated 4-like), also known as RSP5, NEDD4-2 or NEDL3, is a 975 amino acid protein that localizes to the cytoplasm and contains four WW domains, one HECT domain and one C2 domain. Expressed ubiquitously with highest expression in pancreas, prostate and kidney, NEDD4-L functions as an E3 ubiquitin-protein ligase that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme and transfers that ubiquitin residue to substrates targeted for degradation. Through its ability to ubiquitinate and induce the proteasome-dependent degradation of proteins such as Smad2 and TGFβ RII, NEDD4-L is thought to inhibit the TGFβ signaling pathway, thereby regulating the signaling pathways that control cell growth and differentiation. NEDD4-L is expressed as eight isoforms due to alternative splicing events.