PRODUCT CODE: ET1706-07

Recombinant NDUFS3 Monoclonal Antibody (ET1706-07)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

Western blot analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution.
  • Western blot analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution.
  • ICC staining NDUFS3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NDUFS3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin.
Western blot analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NDUFS3 Monoclonal Antibody (ET1706-07)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, MCF-7, PC-3M, human colon cancer tissues, human kidney tissues.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU10-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50 ICC

TARGET

UNIPROT #

PROTEIN NAME

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

GENE NAME

NDUFS3

SYNONYMS

CI-30kD, NDUFS3

SEQUENCE SIMILARITIES

Belongs to the complex I 30 kDa subunit family.

SUBCELLULAR LOCATION

Mitochondrion inner membrane.

FUNCTION

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).