PRODUCT CODE: ET1705-20

Recombinant NDRG1 Monoclonal Antibody (ET1705-20)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NDRG1 on SW480 cell (1) and mouse eye tissue (2) lysate using anti-NDRG1 antibody at 1/1,000 dilution.
  • Western blot analysis of NDRG1 on SW480 cell (1) and mouse eye tissue (2) lysate using anti-NDRG1 antibody at 1/1,000 dilution.
  • ICC staining NDRG1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NDRG1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.
Western blot analysis of NDRG1 on SW480 cell (1) and mouse eye tissue (2) lysate using anti-NDRG1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NDRG1 Monoclonal Antibody (ET1705-20)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480 cell lysate, mouse eye tissue lysate, MCF-7, human colon cancer tissue, human prostate tissue, human placenta tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM32-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

NDRG1

SYNONYMS

42 kDa antibody; Anti GC4 antibody; cap43 antibody; cmt4d antibody; Differentiation related gene1 protein antibody; Differentiation-related gene 1 protein antibody; Drg 1 antibody; DRG-1 antibody; drg1 antibody; gc4 antibody; GC4 antibody; antibody; hmsnl antibody; Human mRNA for RTP complete cds antibody; N myc downstream regulated gene 1 antibody; N myc downstream regulated gene 1 protein antibody; N-myc downstream-regulated gene 1 protein antibody; Ndr 1 antibody; ndr1 antibody; NDRG 1 antibody; Ndrg1 antibody; NDRG1 protein antibody; NDRG1_HUMAN antibody; Nickel specific induction protein antibody; Nickel specific induction protein Cap43 antibody; Nickel-specific induction protein Cap43 antibody; nmsl antibody; Nmyc downstream regulated antibody; Nmyc downstream regulated gene1 antibody; Nmyc downstream regulated gene1 protein antibody; Protein NDRG1 antibody; Protein regulated by oxygen 1 antibody; Protein regulated by oxygen1 antibody; Proxy1 antibody; Reduced in tumor antibody; Reducin antibody; Reducing agents and tunicamycin responsive protein antibody; Reducing agents and tunicamycin-responsive protein antibody; Rit42 antibody; RTP antibody; targ1 antibody; TDD5 antibody; tdds antibody; Tunicamycin responsive protein antibody

SEQUENCE SIMILARITIES

Belongs to the NDRG family.

TISSUE SPECIFICITY

Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Also expressed in heart, brain, skeletal muscle, lung, liver and pancreas. Low levels in peripheral blood leukocytes and in tissues of the immune system. Expressed mainly in epithelial cells. Also found in Schwann cells of peripheral neurons. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.

POST-TRANSLATIONAL MODIFICATION

Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Cell membrane.

FUNCTION

The N-myc downstream regulated gene (NDRG) family is comprised of four members, NDRG1 (also designated Drg1, RTP, rit42, Cap43 and Ndr1), NDRG2, NDRG3 and NDRG4, which share 57-65% homology. The NDRG1 gene, which maps to human chromosome 8q24.22, is evolutionarily conserved and is similarly regulated in humans, mice and rats . Like NDRG2 and NDRG3, NDRG1 is ubiquitously expressed, but it is expressed most prominently in placental membranes and prostate, kidney, small intestine and ovary tissue. NDRG1 gene expression is induced by several compounds, including nickel, and produces a protein involved in stress responses, hormone responses, cell growth and differentiation. The gene encoding NDRG3 maps to human chromosome 20q11.23 and is predominantly expressed in testis, prostate and ovary, which suggests it may play a role in spermatogenesis.