PRODUCT CODE: ET7106-57

Recombinant NCK1 Monoclonal Antibody (ET7106-57)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Hela <br />
 Lane 2: NIH-3T3<br />
 Lane 3: Rat kidney tissue<br />
 Lane 4: Mouse testis tissue
  • Western blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Hela <br />
 Lane 2: NIH-3T3<br />
 Lane 3: Rat kidney tissue<br />
 Lane 4: Mouse testis tissue
  • ICC staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NCK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NCK1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with NCK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH-3T3
Lane 3: Rat kidney tissue
Lane 4: Mouse testis tissue

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant NCK1 Monoclonal Antibody (ET7106-57)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH-3T3, Hela, rat kidney tissue lysate, mouse testis tissue lysate, A431, HUVEC, LOVO, rat testis tissue, human tonsil tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU41-80

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

43/35 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Cytoplasmic protein NCK1

GENE NAME

NCK1

SYNONYMS

Nck-1, NCK1, NCK

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.

SUBCELLULAR LOCATION

Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.

FUNCTION

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.