Applications
-
WB
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Myosin heavy-chain Monoclonal Antibody (ET1702-88)
Immunogen
Recombinant protein with n terminal human myosin heavy chain.
Host
Rabbit
Positive Control
Human skeletal muscle tissue lysates, rat skeletal muscle tissue, rat heart tissue, mouse smooth muscle tissue, mouse skeletal muscle tissue, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF097-7
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
224 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB:1:1,000-1:2,000
-
IHC-P:1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Myosin heavy chain
SYNONYMS
cardiac muscle alpha isoform antibody; MYH6 antibody; MYH6_HUMAN antibody; MYH7 antibody; MYHC antibody; MyHC-alpha antibody; MyHC-beta antibody; MYHCA antibody; MYHCB antibody; Myosin heavy chain 6 antibody; Myosin heavy chain antibody; Myosin heavy chain cardiac muscle alpha isoform antibody; Myosin heavy chain cardiac muscle beta isoform antibody; Myosin-6 antibody
SEQUENCE SIMILARITIES
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
SUBCELLULAR LOCATION
Myofibril.
FUNCTION
Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain by myosin light chain kinase. Myosin heavy chains are encoded by the MYH gene family and have Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains, which were initially isolated from a human fetal skeletal muscle, are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chain are differentially expressed depending on the functional activity of the muscle.