PRODUCT CODE: ET1703-91

Recombinant MyoD1 Monoclonal Antibody (ET1703-91)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of MyoD1 on Hela cells lysates using anti-MyoD1 antibody at 1/1,000 dilution.
  • Western blot analysis of MyoD1 on Hela cells lysates using anti-MyoD1 antibody at 1/1,000 dilution.
  • Flow cytometric analysis of Hela cells with MyoD1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of MyoD1 on Hela cells lysates using anti-MyoD1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MyoD1 Monoclonal Antibody (ET1703-91)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-72

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IP

  • 1:10-1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

Myoblast determination protein 1

GENE NAME

MYOD1

SYNONYMS

bHLHc1, Myf-3, MYOD1

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.; Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).; Ubiquitinated on the N-terminus; which is required for proteasomal degradation.; Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).