PRODUCT CODE: ET1702-15

Recombinant Myelin Basic Protein Monoclonal Antibody (ET1702-15)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Myelin Basic Protein antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Myelin Basic Protein antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Myelin Basic Protein antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Myelin Basic Protein antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Myelin Basic Protein Monoclonal Antibody (ET1702-15)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat brain tissue tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0943

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

33/21/19 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Myelin basic protein

GENE NAME

MBP

SYNONYMS

MBP

SEQUENCE SIMILARITIES

Belongs to the myelin basic protein family.

TISSUE SPECIFICITY

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

DEVELOPMENTAL STAGE

Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.

POST-TRANSLATIONAL MODIFICATION

Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.; The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).; Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.; Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.

SUBCELLULAR LOCATION

Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.; [Isoform 3]: Nucleus. Note=Targeted to nucleus in oligodendrocytes.

FUNCTION

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.