PRODUCT CODE: ET1705-69

Recombinant MVP Monoclonal Antibody (ET1705-69)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of MVP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: A549 cell lysate<br />
 Lane 2: PC-12 cell lysate<br />
 Lane 3: Hela cell lysate<br />
 Lane 4: mouse kidney tissue lysate
  • Western blot analysis of MVP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: A549 cell lysate<br />
 Lane 2: PC-12 cell lysate<br />
 Lane 3: Hela cell lysate<br />
 Lane 4: mouse kidney tissue lysate
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MVP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-MVP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-MVP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of MVP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: mouse kidney tissue lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MVP Monoclonal Antibody (ET1705-69)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549 cell lysate, PC-12 cell lysate, Hela cell lysate, mouse kidney tissue lysate, human colon carcinoma tissue, human stomach carcinoma tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM74-73

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

99 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

MVP

SYNONYMS

LRP antibody; Lung resistance related protein antibody; Lung resistance-related protein antibody; Major vault protein antibody; Major vault protein, rat, homolog of antibody; MVP antibody; MVP_HUMAN antibody; testicular secretory protein Li 30 antibody; VAULT 1 antibody; VAULT1 antibody

TISSUE SPECIFICITY

Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Tyr residues after EGF stimulation.; Dephosphorylated by PTPN11.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Membrane.

FUNCTION

Major vault protein (MVP), is overexpressed in various multidrug-resistant cancer cell lines and clinical samples. The promoter of MVP is TATA-less; contains an inverted CCAAT-box and a Sp1 site located near a p53 binding motif. MVP has two alternative splice variants, which differ from each other within the 5'-leader. The long-MVP isoform is ubiquitously expressed and represents an almost constant portion of the total MVP mRNA in many different normal tissues. MVP is the major component of the multimeric ribonucleoprotein complexes, with several copies of an untranslated RNA, which has been shown to transport along cytoskeletal-based cellular tracks. In conclusion, MVP protein mediates drug resistance, perhaps via a transport process.