PRODUCT CODE: ET1703-99

Recombinant Mre11 Monoclonal Antibody (ET1703-99)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Mre11 on different cells lysates using anti-Mre11 antibody at 1/500 dilution.<br />
Positive control: Line 1: 293T Line 2: Hela <br />
  • Western blot analysis of Mre11 on different cells lysates using anti-Mre11 antibody at 1/500 dilution.<br />
Positive control: Line 1: 293T Line 2: Hela <br />
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- Mre11 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti- Mre11 antibody. Counter stained with hematoxylin.
Western blot analysis of Mre11 on different cells lysates using anti-Mre11 antibody at 1/500 dilution.
Positive control: Line 1: 293T Line 2: Hela

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Mre11 Monoclonal Antibody (ET1703-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, Hela, human breast cancer tissue, human stomach cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-18

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

81 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Double-strand break repair protein MRE11

GENE NAME

MRE11

SYNONYMS

MRE11 homolog 1, MRE11 homolog A, MRE11

SEQUENCE SIMILARITIES

Belongs to the MRE11/RAD32 family.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome.

SUBCELLULAR LOCATION

Nucleus. Chromosome, telomere. Chromosome. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents.

FUNCTION

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.