PRODUCT CODE: ET1610-65

Recombinant Moesin Monoclonal Antibody (ET1610-65)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Moesin on different lysates using anti-Moesin antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: Raji <br />
  Lane 2: SH-SY-5Y
  • Western blot analysis of Moesin on different lysates using anti-Moesin antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: Raji <br />
  Lane 2: SH-SY-5Y
  • ICC staining Moesin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Moesin in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Moesin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Moesin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Moesin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Moesin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Moesin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Moesin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Moesin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Moesin on different lysates using anti-Moesin antibody at 1/1,000 dilution.
Positive control:
Lane 1: Raji
Lane 2: SH-SY-5Y

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Moesin Monoclonal Antibody (ET1610-65)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Raji cell lysate, SH-SY5Y cell lysate, Hela, NCCIT, human tonsil tissue, human spleen tissue, human breast carcinoma tissue, mouse lung tissue, mouse placenta tissue, mouse stomach tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC69-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Moesin

SYNONYMS

Epididymis luminal protein 70 antibody; HEL70 antibody; Membrane organizing extension spike protein antibody; Membrane-organizing extension spike protein antibody; MOES_HUMAN antibody; Moesin antibody; Moesin/anaplastic lymphoma kinase fusion protein antibody; Msn antibody; MSN/ALK fusion antibody

TISSUE SPECIFICITY

In all tissues and cultured cells studied.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity). Phosphorylation on Thr-558 by STK10 negatively regulates lymphocyte migration and polarization.; S-nitrosylation of Cys-117 is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) implicating the iNOS-S100A8/9 transnitrosylase complex.

SUBCELLULAR LOCATION

Cell membrane, Apical cell membrane, microvillus membrane, cytoskeleton, microvillus.

FUNCTION

Ezrin, Moesin and Radixin belong to a family of highly homologous Actin-associated proteins that are localized just beneath the plasma membrane. These proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share more than 70% homology with Ezrin and are co-expressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.